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Product Details
Synonyms
Flt-3, Flk-2, STK-1, CD135, FLK2, FLT-3
Source
Mouse Flt-3, His Tag (FL3-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 28 - Ser 544 (Accession # NP_034359.2).
Predicted N-terminus: Asn 28
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse Flt-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Mouse Flt-3, His Tag (Cat. No. FL3-M52H6) at 5 μg/mL (100 μL/well) can bind CD135 (FLT3) Antibody, Anti-mouse with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Mouse Flt-3, His Tag (Cat. No. FL3-M52H6) immobilized on CM5 Chip can bind Mouse Flt-3 Ligand, His Tag (Cat. No. FLL-M52H4) with an affinity constant of 59.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tyrosine-protein kinase receptor FLT3
- Category：
- Listed Drugs Count：
14 Details
- Clinical Drugs Count：
59 Details
- Highest R&D Stage：
Approved
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