Mouse FGL1 Protein, Fc Tag

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Cat. No. / Size
Price
Qty
FG1-M5258-100ug
$360.00
FG1-M5258-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    FGL1, Hepassocin, HP-041, HFREP-1, LFIRE-1, HFREP1

  • Source

    Mouse FGL1, Fc Tag (FG1-M5258) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Ile 314 (Accession # Q71KU9-1).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    FGL1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 60.5 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FGL1 SDS-PAGE

    Mouse FGL1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     FGL1 ELISA

    Immobilized Mouse LAG-3, His Tag (Cat. No. LA3-M52H5) at 5 μg/mL (100 μL/well) can bind Mouse FGL1, Fc Tag (Cat. No. FG1-M5258) with a linear range of 0.008-0.5 μg/mL (QC tested).

    Protocol

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Background

Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.

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