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Product Details
Synonyms
FCGR1A, FCG1, FCGR1, IGFR1, CD64, CD64A, FCRI
Source
Rat CD64 Protein, His Tag (CD4-R52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 285 (Accession # A0A0B4J2J0).
Predicted N-terminus: Gln 16
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32.1 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Rat CD64 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat CD64 Protein, His Tag (Cat. No. CD4-R52H3) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (Cat. No. GMP-MC0323) immobilized on CM5 Chip can bind Rat CD64 Protein, His Tag (Cat. No. CD4-R52H3) with an affinity constant of 849 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
High affinity immunoglobulin gamma Fc receptor I
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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