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Product Details
Synonyms
Ephrin type-A receptor 5
Source
Human EphA5, His Tag (EP5-H52H5) is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Pro 573 (Accession # P54756-1).
Predicted N-terminus: Pro 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 62.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human EphA5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human EphA5, His Tag (Cat. No. EP5-H52H5) is more than 95% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.
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Bioactivity-SPR
Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human EphA5, His Tag (Cat. No. EP5-H52H5) with an affinity constant of 0.576 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
EPH Receptor A5
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 2 Clinical
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