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Product Details
Product Details
This product is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 110 kDa.The enzyme deglycosylates IgG after the core GlcNAc and display limited activity on high-mannose and hybrid-type glycans.
Application
Endo S2 is specific for N-glycans attached on the Fc-domain of IgGs, and hydrolyzes Fc-glycans of all human IgG subclasses and IgG from many other species, including mouse, rat, monkey, sheep, goat, cow, and horse.
Unit Definition
One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.
Enzyme Activity
>200 U/μL
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt.
- -70°C for 12 months under sterile conditions.
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Bioactivity
100 unit Endo S2 deglycosylates ≥ 95% of 100 µg IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min (QC tested).
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