Endo S2 (200U/μL)

This product is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 110 kDa.The enzyme deglycosylates IgG after the core GlcNAc and display limited activity on high-mannose and hybrid-type glycans.

Endo S2 is specific for N-glycans attached on the Fc-domain of IgGs, and hydrolyzes Fc-glycans of all human IgG subclasses and IgG from many other species, including mouse, rat, monkey, sheep, goat, cow, and horse.

One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.

>90% as determined by SDS-PAGE.

>200 U/μL

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

Contact us for customized product form or formulation.

This product is supplied and shipped on dry ice.

• The product MUST be stored at -70°C or lower upon receipt.
• -70°C for 12 months under sterile conditions.