Human E-Cadherin / Cadherin-1 (155-709) Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
ECD-H5256-100ug
$285.00
ECD-H5256-1mg (250ug X 4)
$1815.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Cadherin-1, CDH1, CD324, E-cadherin, Arc-1, ECAD, LCAM, UVO

  • Source

    Human E-Cadherin (155-709) Protein, Fc Tag (ECD-H5256) is expressed from human 293 cells (HEK293). It contains AA Asp 155 - Ala 709 (Accession # P12830-1).

    Predicted N-terminus: Asp 155

    Request for sequence
  • Molecular Characterization

    E-Cadherin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 87.0 kDa . The protein migrates as 50 kDa and 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    E-Cadherin SDS-PAGE

    Human E-Cadherin (155-709) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    E-Cadherin SEC-MALS

    The purity of Human E-Cadherin (155-709) Protein, Fc Tag (Cat. No. ECD-H5256) is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     E-Cadherin ELISA

    Immobilized Mouse KLRG1, His Tag (Cat. No. KL1-M5249) at 5 μg/mL (100 μL/well) can bind Human E-Cadherin (155-709) Protein, Fc Tag (Cat. No. ECD-H5256) with a linear range of 0.02-1.25 μg/mL (QC tested).

    Protocol

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Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).

Recent Advances

 
Drug Development Progress
  • English Name:

    Cadherin-1

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Preclinical

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