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Product Details
Synonyms
DNAM1, CD226, PTA1
Source
Cynomolgus DNAM-1, His Tag (DN1-C52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Phe 252 (Accession # A0A2K5W1Z6-1).
Predicted N-terminus: Glu 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.5 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus DNAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 2-40 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) at 2 μg/mL (100 μL/well) can bind Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) with a linear range of 5-40 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) with an affinity constant of 0.88 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
CD226 antigen
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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