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Product Details
Synonyms
Coagulation factor VII, F7, SPCA, Proconvertin
Source
Mouse Coagulation factor VII, His Tag (COI-M52H1) is expressed from human 293 cells (HEK293). It contains AA Ala 42 - Leu 446 (Accession # P70375-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.7 kDa. The protein migrates as 57-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM Nacl,pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse Coagulation factor VII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse Coagulation factor VII, His Tag (Cat. No. COI-M52H1) is more than 90% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.
Report
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >9 pmol/min/μg (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Coagulation factor VII
- Category：
- Listed Drugs Count：
21 Details
- Clinical Drugs Count：
10 Details
- Highest R&D Stage：
Approved
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