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Product Details
Synonyms
CD3 epsilon & CD3 gamma, CD3E & CD3G
Source
APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDG-HA2W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 & Gln 23 - Ser 116 (Accession # P07766-1 & P09693-1). It is the APC labeled form of Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9).
Predicted N-terminus: Asp 23 & Gln 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). The subunit CD3E carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The subunit CD3G carries a human IgG1 Fc fragment at the C-terminus, followed by a flag tag.
Conjugate
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA
Immobilized APC-Labeled Human CD3E & CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-HA2W3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) (Cat. No. CDE-M532) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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FAQ
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Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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