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PE-Labeled Human CD3E & CD3D Protein, His,Avitag™Star Staining

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Product Number/Spec
Price
Menge
CDD-HP2E3-25ug
$480.00
CDD-HP2E3-200ug
$3270.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    CD3E & CD3D, CD3 delta & CD3 epsilon

  • Source

    PE-Labeled Human CD3E & CD3D Protein, His,Avitag (CDD-HP2E3) is produced via conjugation of PE to Human CD3E&CD3D Protein, His,Avitag with a new generation site-specific technology under Star Staining labeling platform. Human CD3E&CD3D Protein, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).

    Predicted N-terminus: Asp 23 & Phe 22

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • Bioactivity-FACS

     CD3E & CD3D FACS

    1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of PE-Labeled Human CD3E & CD3D Protein, His,Avitag (Cat. No. CDD-HP2E3) and negative control protein respectively, PE signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  CD3E & CD3D FACS

    1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of PE-Labeled Human CD3E & CD3D Protein, His,Avitag (Cat. No. CDD-HP2E3) and negative control protein respectively, PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Publish Comment

Background Introduction

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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