Biotinylated Human CD3 epsilon Protein, His Tag, ultra sensitivity (primary amine labeling)

分子别名（Synonym）

FLJ18683,T3E,TCRE,CD3E,CD3-epsilon

表达区间及表达系统（Source）

MABSol® Biotinylated Human CD3E, His Tag, primary amine labeling (CDE-H8223) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).

Predicted N-terminus: Asp 23

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 12.6 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).

标记（Biotinylation）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度（Biotin:Protein Ratio)

The biotin to protein ratio is 2-3 as determined by the HABA assay.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by reduced SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 12 months under sterile conditions after reconstitution.