Human BTN3A1 / CD277 Protein, Fc Tag (MALS verified)

Product Number/Spec
Price
Availability
Menge
BT1-H5255-100ug
$355.00
In Stock - Delivery in 1-2 business days
BT1-H5255-1mg (500ug X 2)
$2,140.00
In Stock - Delivery in 1-2 business days
BT1-H5255
Product Amount :
$ 0
0
Add to Cart
New Product Suggestion Request Quote
Contact Us
Contact Us
Product Amount:
$ 0
0
Add to Cart
  • Product Details
  • Data Display
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

BTN3A1, CD277, BTF5

Source

Human BTN3A1, Fc Tag (BT1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Gly 254 (Accession # O00481-1).

Predicted N-terminus: Gln 30

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 50.8 kDa. The protein migrates as 58-65 kDa under reducing (R) condition, and 120 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.