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Product Details
Synonyms
ACE-2, ACEH, ACE2
Source
Mink ACE2, His Tag (AC2-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Thr 740 (Accession # QPL12211.1).
Predicted N-terminus: Gln 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 86.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM Nacl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mink ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mink ACE2, His Tag (Cat. No. AC2-M52H3) is more than 85% and the molecular weight of this protein is around 175-195 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Mink ACE2, His Tag (Cat. No. AC2-M52H3) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD (Y453F), His Tag with an affinity constant of 0.178 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Mink ACE2, His Tag (Cat. No. AC2-M52H3) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 8.34 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Angiotensin-converting enzyme 2
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
7 Details
- Highest R&D Stage：
Phase 2 Clinical
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