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Product Details
Product Details
This Laminin 521 Protein Screening Panel consists of three distinct laminin 521 proteins designed to identify the optimal substrate for stem cell culture applications, including proliferation and differentiation. Performance may vary across different stem cell lines. A coating procedure can be carried out in plates or flasks to screen and select the most suitable laminin 521 protein for specific experimental requirements.
Components
Cat. No.NameSizeLA5-H5248Human Laminin 521 Protein, premium grade (Flagship)100 μgLA5-H5213LHuman Laminin 521 Protein, premium grade (Liquid)100 μgLA5-H5217Human Laminin 521 Protein, premium grade (Flagship Pro)100 μgKey parameter
Purity (SDS PAGE)> 90%Mycoplasma TestNegativeSterility TestNegativeEndotoxin Test<0.5 EU per μgFormulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with protectants.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -70°C for 2 years under sterile conditions;
- 2-8°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 521 Protein, premium grade (Flagship Pro) (Cat. No. LA5-H5217) with a linear range of 0.1-3 ng/mL (QC tested).
ProtocolBioactivity-SPR
Human Laminin 521 Protein, premium grade (Flagship) (Cat. No. LA5-H5248) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 1.09 nM as determined in a SPR assay (Biacore 8K) (QC tested).
ProtocolBioactivity-BLI
Loaded Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) on HIS1K Biosensor, can bind Human Laminin 521 Protein, premium grade (Liquid) (Cat. No. LA5-H5213L) with an affinity constant between 0.50 nM - 5.00 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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