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Assay Type | Sandwich-ELISA |
Analyte | Vitronectin |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | <0.375 ng/mL |
Standard Curve Range | 0.375 ng/mL-12 ng/mL |
Assay Time | 3 h 20 min |
Suitable Sample Type | For the detection and quantitative determination of Vitronectin in stem cell culture supernates, serum, plasma and cell lysates. |
Sample volume | 100 μL |
Vitronectin, a key extracellular matrix glycoprotein, exerts crucial effects on stem cell adhesion, proliferation, differentiation, self-renewal maintenance, cell survival, and integrin-mediated signaling. It exists as a monomeric glycoprotein with a modular structure, containing functional domains that bind to integrins (e.g., αvβ3, αvβ5), heparin, and other matrix components. Different isoforms, including plasma-derived and cell-secreted vitronectin, play distinct roles in stem cell niche regulation, tissue regeneration, and cell-matrix crosstalk—particularly critical for maintaining stem cell pluripotency and guiding directed differentiation.
To support the advancement of stem cell therapy and regenerative medicine research, ACROBiosystems developed Human Vitronectin Quantitative ELISA Kit with strict methodological validation. This kit is specifically designed for the detection and quantitative determination of vitronectin in stem cell culture supernates, serum, plasma, and cell lysates. Additionally, it can be used for the quantitative analysis of Recombinant Vitronectin, premium grade (ACROBiosystems, cat# GMP-VINH19) concentrations—an essential tool for quality control of stem cell culture supplements and evaluation of vitronectin-mediated stem cell behavior.
The kit is developed for the detection and quantitative determination of Vitronectin in cell culture supernates.
It is for research use only.
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
ID | Components | Size |
RES103-C01 | Pre-coated Anti-Vitronectin Antibody Microplate | 1 plate (8×12 strips) |
RES103-C02 | Human Vitronectin Standard | 10 μg×2 |
RES103-C03 | Biotin-Anti-Vitronectin Antibody | 20 μg |
RES103-C04 | Streptavidin-HRP | 50 μL |
RES103-C05 | 10×Washing Buffer | 50 mL |
RES103-C06 | 2×Dilution Buffer | 50 mL |
RES103-C07 | Substrate Solution | 12 mL |
RES103-C08 | Stop Solution | 7 mL |