resDetect™ Vitronectin ELISA Kit (Residue Testing)

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RES-A103-96tests
$294.00
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RES-A103
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Product Specifications

Assay Type
Sandwich-ELISA
Analyte
Vitronectin
Format
96T (8×12 strips)
Reactivity
Human
Regulatory Status
RUO
Sensitivity
<0.375 ng/mL
Standard Curve Range
0.375 ng/mL-12 ng/mL
Assay Time
3 h 20 min
Suitable Sample Type
For the detection and quantitative determination of Vitronectin in stem cell culture supernates, serum, plasma and cell lysates.
Sample volume
100 μL

Product Overview

Vitronectin, a key extracellular matrix glycoprotein, exerts crucial effects on stem cell adhesion, proliferation, differentiation, self-renewal maintenance, cell survival, and integrin-mediated signaling. It exists as a monomeric glycoprotein with a modular structure, containing functional domains that bind to integrins (e.g., αvβ3, αvβ5), heparin, and other matrix components. Different isoforms, including plasma-derived and cell-secreted vitronectin, play distinct roles in stem cell niche regulation, tissue regeneration, and cell-matrix crosstalk—particularly critical for maintaining stem cell pluripotency and guiding directed differentiation.​

To support the advancement of stem cell therapy and regenerative medicine research, ACROBiosystems developed Human Vitronectin Quantitative ELISA Kit with strict methodological validation. This kit is specifically designed for the detection and quantitative determination of vitronectin in stem cell culture supernates, serum, plasma, and cell lysates. Additionally, it can be used for the quantitative analysis of Recombinant Vitronectin, premium grade (ACROBiosystems, cat# GMP-VINH19) concentrations—an essential tool for quality control of stem cell culture supplements and evaluation of vitronectin-mediated stem cell behavior.

Application

The kit is developed for the detection and quantitative determination of Vitronectin in cell culture supernates.

It is for research use only.

Storage

1. Store the unopened kit at 2-8°C upon receipt.

2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

Materials Provided

ID
Components
Size
RES103-C01
Pre-coated Anti-Vitronectin Antibody Microplate
1 plate (8×12 strips)
RES103-C02
Human Vitronectin Standard
10 μg×2
RES103-C03
Biotin-Anti-Vitronectin Antibody
20 μg
RES103-C04
Streptavidin-HRP
50 μL
RES103-C05
10×Washing Buffer
50 mL
RES103-C06
2×Dilution Buffer
50 mL
RES103-C07
Substrate Solution
12 mL
RES103-C08
Stop Solution
7 mL