Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteVCAM-1 (Fc Tag)Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity<0.469 ng/mLStandard Curve Range0.469 ng/mL-15 ng/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of VCAM-1 (Fc Tag) in cell culture supernates.Sample volume100 μLProduct Overview
resDetect™ VCAM-1(Fc Tag) ELISA kit (Residue Testing)is based on the ELISA sandwich method and is used to detect and quantitatively determine Human VCAM-1 residues in cell culture supernatants. The kit GMP Human VCAM-1 Protein, Fc Tag (ACROBiosystems, cat# GMP-VC1H25) to ensure accurate assay results and is designed to provide a reliable solution for cell therapy product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human VCAM-1.
Application
The kit is developed for the detection and quantitative determination of VCAM-1 (Fc Tag) in cell culture supernates.
It is for research use only.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES101-C01Pre-coated Anti-Human VCAM-1 Antibody Microplate1 plate (8×12 strips)RES101-C02Human VCAM-1 (Fc Tag) Standard15 μgRES101-C03Biotin-Anti-Human VCAM-1 Antibody15 μgRES101-C04Streptavidin-HRP50 μLRES101-C0510×Washing Buffer50 mLRES101-C062×Dilution Buffer50 mLRES101-C07Substrate Solution12 mLRES101-C08Stop Solution7 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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