Mouse TNF-alpha Detection Kit (TR-FRET)AIDD‑Ready

Customer Reviews
Cat. No. / Size
Price
Availability
Qty
FRT-B102-100tests
$450.00
In Stock - Delivery in 2-3 weeks
FRT-B102-500tests
$780.00
In Stock - Delivery in 2-3 weeks
FRT-B102-10000tests (500tests X 20)
$7800.00

Product Details

  • FAB

    1. Comprehensive validation - Rigorously validated through comprehensive methodology to ensure reliable and accurate results.
    2. Simple and fast operation - No complicated washing steps, significantly reducing time.
    3. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
    4. Accurate and reliable results - High sensitivity with minimal matrix effects.
    5. High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
    6. Fast completion - Results in just 2 hours.
  • Product Overview

    Mouse TNF-alpha Detection Kit (TR-FRET) enables sensitive and rapid detection of mouse TNF-alpha in a homogeneous system within 2 hours. Designed for ease of use and high-throughput screening, it is fully compatible with microplate formats, allowing efficient analysis of large sample volumes. This kit is specifically designed for the accurate quantitation of mouse TNF-alpha in cell culture supernatant, serum, and plasma.

  • Product Specifications

    Assay Type
    Sandwich-TR-FRET
    Analyte
    Mouse TNF-alpha
    Reactivity
    Mouse
    Regulatory Status
    RUO
    Sensitivity
    LOQ=39.063 pg/mL
    Standard Curve Range
    39.063 pg/mL-5000 pg/mL
    Assay Time
    2 hr
    Suitable Sample Type
    For Mouse TNF-alpha detection in human serum, human plasma,cell culture supernatants
    Sample volume
    16 μL

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Workflow

    TNF-alpha Workflow

  • Storage

    1. Store the unopened kit at 2-8°C upon receipt.

    2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

    3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    FRTB102-C01
    Anti-Mouse TNF-alpha Antibody Europium-chelate
    100 tests/500 tests
    FRTB102-C02
    FA Labeled Anti-Mouse TNF-alpha Antibody
    100 tests/500 tests
    FRTB102-C03
    Mouse TNF-alpha Standard
    5 μg/100 tests
    5 μg/500 tests
    DB-04
    TR-FRET Sample Dilution Buffer, pH7.4
    50 mL/100 tests & 500 tests
    DB-05
    TR-FRET Detection Buffer, pH7.4
    50 mL/100 tests & 500 tests
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

Validation
  • Dilution Linearity

    To assess the linearity of the assay, samples spiked with high concentrations of mouse TNF-alpha standard were serially diluted with TR-FRET Sample Dilution Buffer, pH 7.4 (DB-04), to generate samples with concentrations within the dynamic range of the assay.

     TNF-alpha DILUTION LINEARITY
  • Intra-Assay Statistics

    Five samples with known concentrations were tested repeatedly 16 times within a single analytical batch to assess within-batch precision and accuracy, Intra-Assay Precision CV≤30%, Intra-Assay Accuracy Recovery: 70%-130%.

     TNF-alpha INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Five samples with known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy. Inter-Assay Precision CV≤30%, Inter-Assay Accuracy Recovery: 70%-130%.

     TNF-alpha INTER-ASSAY STATISTICS

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