Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay Kit (Spike RBD)

背景（Background）

Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant B.1.351 identified in South Africa shows evidence of increased transmissibility and resistance to established immunity. There are 10 mutations on the spike protein of the South African variant (L18F, D80A, D215G, LAL242-244del, R246I, K417N, E484K, N501Y, D614G, A701V); of which 3 (K417N, E484K, N501Y) resides on the receptor-binding domain (RBD). To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.

应用说明（Application）

This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike RBD antibody in serum/plasma in vitro. The Spike RBD antigen used in this kit contains K417N, E484K, N501Y mutations found in the South African variant (known as B.1.351 or 20C/501Y.V2).

It is for research use only.

存储 & 运输（Storage & Shipping）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理（Assay Principles）

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.