Product Details
Materials Provided
IDComponentsSizeEP107-C01Pre-coated Human ACE2 Microplate1 plateEP107-C02SARS-CoV-2 Inhibitor10 μgEP107-C03HRP-SARS-CoV-2 Spike RBD(WT)15 μgEP107-C0410xWashing Buffer50 mLEP107-C05Dilution Buffer50 mLEP107-C06Substrate Solution12 mLEP107-C07Stop Solution7 mLProduct Overview
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. The deleloped of effcient treatment agaginst SARS-CoV-2 is an emergent need for global pubic heath,and it is necessary to develop SARS-CoV-2 inhibitors, such as vaccines, therapeutic antibodies and small molecule compounds against SARS-CoV-2.
Therefore, it is helpful to develop the SARS-CoV-2 (WT) Inhibitor Screening Kit (Spike RBD) to test SARS-CoV-2 inhibitors.
Application
This kit is developed for screening inhibitors of SARS-CoV-2 (WT).
It is for research use only.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Data Display
Typical Data
Please refer to DS document for the assay protocol.

Incubation of SARS-CoV-2 inhibitor:Blocking the binding of HRP-SARS-CoV-2 Spike RBD(WT) to Human ACE2.
Serial dilutions of SARS-CoV-2 inhibitor (Catalog # EP107-C02) (1:1 serial dilutions, from 5.0ug/mL to 0.0098ug/mL(34.4nM to 0.07nM)) and loaded onto the plate coated by human ACE2 in the presence of HRP-SARS-CoV-2 Spike RBD(WT).Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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