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Product Details
Product Overview
The Monkey GDF‑15 ELISA Kit is a ready‑to‑use immunoassay kit specifically designed for the quantification of native and recombinant monkey GDF‑15 in biological samples, including serum and plasma. Our ELISA kit offers multiple advantages:
Product Specifications
Assay TypeSandwich-ELISAAnalyteGDF-15Format96-wells plate breakable into 12 x 8 wells stripsReactivityMonkeySensitivity5 pg/mLRange11.72 pg/mL-750 pg/mLAssay Time2.75 hSample volume50 μLSample TypeFor the quantitative determination of Monkey GDF-15 in serum, plasma.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for the quantification of native and recombinant monkey GDF‑15 in serum, plasma.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA297-C01Pre-coated Anti-GDF-15 Antibody Microplate1 plateCEA297-C02Monkey GDF-15 Standard5 μg/mL×2CEA297-C03Biotin-Anti-GDF-15 Antibody Con. Solution100 μLCEA297-C04Biotin-Antibody Dilution Buffer8 mLCEA297-C05Streptavidin-HRP Con. Solution500 μLCEA297-C06Streptavidin-HRP Dilution Buffer15 mLCEA297-C0720× Washing Buffer50 mLCEA297-C08Sample Dilution Buffer15 mL×2CEA297-C09Substrate Solution12 mLCEA297-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Monkey GDF-15 is less than 5 pg/mL.
Intra-Assay Statistics
Ten replicates of each of 3 samples containing different GDF-15 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of GDF-15 were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant GDF-15 was spiked into 3 Monkey serum samples, and then analyzed. The average recovery of GDF-15 for serum samples is 111.2%.
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