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Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-7Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜6.25 pg/mLStandard Curve Range6.25 pg/mL-200 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the quantitative determination of human IL-7 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 μLProduct Overview
Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-7 residues in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-7 (ACROBiosystems, cat# GMP-L07H24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human Interleukin-7 (IL-7).
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES055-C01Pre-coated Anti-IL-7 Antibody Microplate1 plate (8×12 strips)RES055-C02Human IL-7 Standard20 μgRES055-C03Biotin-Anti-IL-7 Antibody20 μgRES055-C04Streptavidin-HRP50 μLRES055-C0510xWashing Buffer50 mLRES055-C062xDilution Buffer50 mLRES055-C07Substrate Solution12 mLRES055-C08Stop Solution7 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
User Reviews Publish Comment
Frontier Progress
Drug Development Progress- English Name：
Interleukin-7
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
5 Details
- Highest R&D Stage：
Phase 1 Clinical
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