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Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteHGFFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜62.5 pg/mLStandard Curve Range62.5 pg/mL-2000 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of HGF in cell culture supernates.Sample volume100 μLProduct Overview
resDetect™ HGF ELISA kit (Residue Testing)is based on the ELISA sandwich method and is used to detect and quantitatively determine Human HGF residues in cell culture supernatants. The kit GMP Human HGF Protein (ACROBiosystems, cat# GMP-HGFH28) to ensure accurate assay results and is designed to provide a reliable solution for cell therapy product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human HGF.
Application
The kit is developed for the detection and quantitative determination of HGF in cell culture supernates.
It is for research use only.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES145-C01Pre-coated Anti-HGF Antibody Microplate1 plate (8×12 strips)RES145-C02Human HGF Standard20 μgRES145-C03Biotin-Anti-HGF Antibody20 μgRES145-C04Streptavidin-HRP50 μLRES145-C0510×Washing Buffer50 mLRES145-C06Dilution Buffer50 mLRES145-C07Substrate Solution12 mLRES145-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve should be generated for each microplate. The absolute OD values may vary depending on the laboratory, operator, or equipment. The example data provided below are for reference only.
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