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Product Specifications
Assay TypeSandwich-ELISAAnalyteFibronectinFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜100 pg/mLStandard Curve Range100 pg/mL-3200 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of Human Fibronectin in cell culture supernates.Sample volume100 uLProduct Overview
Fibronectin, a vital extracellular matrix protein, significantly impacts cell adhesion, spreading, migration, tissue repair, cell shape maintenance, and cell signaling. It exists as a dimer with disulfide - linked polypeptide chains and multiple functional domains. Different isoforms, like soluble and cell - associated ones, play distinct roles in wound healing, blood clotting, and cell - matrix interactions.
To support the development of cell therapy drugs, ACROBiosystems developed Human fibronectin quantitive ELISA Kit with rigorous methodological validation, which is used for the detection and quantitative determination of fibronectin in cell culture supernates, serum, and plasma. Besides, this kit can also be used for the quantitative determination of Recombinant Fibronectin fragment, premium grade (ACROBiosystems, cat# GMP-FINH18) concentrations.
Application
The kit is developed for the detection and quantitative determination of fibronectin in cell culture supernates.
It is for research use only.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES108-C01Pre-coated Anti-Human Fibronectin Antibody Microplate1 plate(8×12 strips)RES108-C02Human Fibronectin Standard5 μg×2RES108-C03Biotin-Anti-Human Fibronectin Antibody20 μgRES108-C04Streptavidin-HRP50 μLRES108-C0510×Washing Buffer50 mLRES108-C062×Dilution Buffer50 mLRES108-C07Substrate Solution12 mLRES108-C08Stop Solution7 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Fibronectin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Recovery
Five parts of blank T cell culture supernatant were added with different concentrations of human Fibronectin, and the T cell culture supernatant without human Fibronectin was used as background to calculate the recovery rate. The range of the recovery rate is 82.5-93.7%, and the average recovery is 89.3%.
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