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Materials Provided
IDComponentsSizeAAVX01-C01AAV Capsid Capture Antibody120 μgAAVX01-C02AAV Capsid Detection Antibody60 μgAAVX01-C03HRP-Conjugated Antibody15 μgApplication
AAV Titration Detection Kit (Universal )was developed for the detection and quantitative determination of AAV capsid titration in AAV gene therapy product preparation processing.
It is for research use only.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
The unopened kit is stable for 36 months from the date of manufacture if stored at -20°C to -70°C.
The opened kit should be stored per components table. The shelf life is 90 days from the date of opening.
ACRO Quality Management System
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Typical Data
Please refer to DS document for the assay protocol.
Immobilized AAV Capsid Capture Antibody (AAVX01-C01) at 1.0 μg/mL (100 μL/well) can bind AAV2 Capsid, and then add AAV Capsid Detection Antibody (AAVX01-C02) at 0.5 μg/mL (100 μL/well), final detection was performed using HRP-Conjugated Antibody (AAVX01-C03) with a linear range of 3.91E+07 - 2.50E+09 capsids/mL (QC tested).
Immobilized AAV Capsid Capture Antibody (AAVX01-C01) at 1.0 μg/mL (100 μL/well) can bind AAV Capsid, and then add AAV Capsid Detection Antibody (AAVX01-C02) at 0.5 μg/mL (100 μL/well), final detection was performed using HRP-Conjugated Antibody (AAVX01-C03).
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