Human TSHR (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human TSHR (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human TSHR (Uniprot: P16473-1). When stimulated with human TSH protein or TSHR agonist antibody, receptor-mediated signaling can drive CREB-mediated luminescence. Neutralization of biological effect of human TSH protein by corresponding antibody results in a decrease in luminescence.

• Screen for anti-human TSHR agonist antibody.

• Screen for neutralizing antibodies blocking the stimulation of human TSH protein.

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

DMEM medium + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.