HEK293/Human GPC3 ΔHS Stable Cell Line

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The HEK293/Human GPC3 ΔHS Stable Cell Line was engineered to express the human GPC3 ΔHS lacking the heparan sulfate (HS) chains, which produced by introducing the point mutations (S495A and S509A) on the wild-type human GPC3 (Uniprot: P51654-1). Surface expression of human GPC3 ΔHS was confirmed by flow cytometry.

• Useful for cell-based GPC3 ΔHS binding assay

Adherent

Puromycin (2 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.