Human GLP-1R (GFP) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The GLP-1R (GFP) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human GLP-1R (Uniprot: P43220), which can drive GFP expressing systems by GLP-1R agonists or glucagon-like peptide 1 (GLP-1) stimulation. In the absence of agonist or GLP-1, the GLP-1R receptor is not activated and fluorescence signal is low. In the presence of agonist or GLP-1, the GLP-1R pathway-activated fluorescence can be detected in a dose-dependent manner.

• Screen for agonists that can bind and activate GLP-1R

Adherent

Puromycin(2 μg/mL) + Hygromycin B(20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.