Human 5-HT1A (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human 5-HT1A (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human 5-HT1A (Uniprot: P08908) and mGFP by a viral P2A self-cleaving peptide. When stimulated with human 5-HT1A agonist, the agonist/5-HT1A interaction drives CREB-mediated luminescence. Inhibition of activated 5-HT1A signaling by corresponding inhibitors results in a decrease in luminescence.

• Screen for 5-HT1A agonist.

• Screen for 5-HT1A antagonist.

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.