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Product Details
- ProductSizeAmountActiveMax® Human MSLN μBeads, premium grade (for cells)2.5 mg2.5×10⁷ beadsActiveMax® Human MSLN μBeads, premium grade (for cells)10 mg (2.5 mg×4)1.0×10⁸ beads
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This product can be directly isolated using conventional column-free magnetic stand (eg. Cat. MB-01/ MB-02/ MB-03/ MB-04). Please note that due to its micron-scale size, it is not compatible with commercially available column-based (M/D/L) magnetic separation systems.
Product Description
ActiveMax® Human MSLN μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human MSLN protein expressed from human 293 cells (HEK293) and contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
ActiveMax® Human MSLN μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.Application
ActiveMax® Human MSLN μBeads, premium grade (for cells) are designed to stimulate in vitro MSLN-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human MSLN antigen. It can be used as follows:
Evaluating the characteristics of CAR-T cells or UCAR-T cells.
In vitro expansion of MSLN-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of MSLN-specific CAR-T cells or UCAR-T cells.Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please protect from light and avoid repeated freeze-thaw cycles.Immediate use after reconstitution is highly recommended.
Sterility
Negative
Endotoxin
Less than 0.002 EU per μg by the LAL method / rFC method.
Mycoplasma
Negative
Important Note
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Formulation
Lyophilized in PBS with 0.1% HSA, pH 7.4. Trehalose is added as protectant before lyophilization.
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Performance Data
FACS Data
Assay of human MSLN protein on the μBeads surface by Flow cytometry. ActiveMax® Human MSLN μBeads, premium grade (for cells) (Cat. No. MBS-C003) were stained with anti-human MSLN antibody, and then followed by FITC anti-human IgG Fc antibody and analyzed by flow cytometry.
Protocol
Stimulate Mesothelin CAR-T Cells -Cytokines
ActiveMax® Human MSLN μBeads, premium grade (for cells) (Cat. No. MBS-C003) can activate MSLN-specific CAR-T cells by detecting the secretion of IFN- γ in vitro.
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h, the cell-free supernatant was collected by centrifugation for evaluating IFN-γ, TNFα and IL-4 secretion by CBA. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the IFN-γ, TNFα and IL-4 increased (Data is the average of 3 independent experiments, error bars ± SD).
Stimulate Mesothelin CAR-T Cells -Cytotoxic Molecules
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h, the cell-free supernatant was collected by centrifugation for evaluating Granzyme B secretion by ELISA. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the Granzyme B increased (Data is the average of 3 independent experiments, error bars ± SD).
Stimulate Mesothelin CAR-T Cells -Activation Marker
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h. After demagnetization of the beads, the cells were collected and CD25, CD69, and CD137 were detected by FACS. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the three markers increased (Data is the average of 3 independent experiments, error bars ± SD).
Stimulate Mesothelin CAR-T Cells -Exhaustion Marker
MSLN CAR-T cells were stimulated with ActiveMax® Human MSLN μBeads (Cat. No. MBS-C003) for 24h. After demagnetization of the beads, the cells were collected and LAG3, PD-1 were detected by FACS. The results indicated that MSLN CAR-T cells responded to MSLN μBeads, and the expressions of the two markers increased (Data is the average of 3 independent experiments, error bars ± SD).
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Mesothelin
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