Product Details
Source
Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
1D10
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Human enterovirus 71
Immunogen
Recombinant Human enterovirus 71 (strain USA/BrCr/1970) (EV71) VP0 Protein is expressed from Baculovirus-Insect cells
Specificity
Specifically recognizes EV71- VP0 Protein.
Application
ApplicationRecommended UsageWestern Blot0.1-10 ug/mLELISA0.2-2 μg/mLPurification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA

Immobilized Human enterovirus 71 (strain USA/BrCr/1970) (EV71) VP0 Protein, His Tag (Cat. No. VP0-V5244) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10) (Cat. No. VP0-MY321) with a linear range of 0.04-0.8 ng/mL (QC tested).
Protocol
Western Blot

Detection of Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10), Human IgG1 | Human Kappa, HEK by Western Blot. Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10), Human IgG1 | Human Kappa, HEK at 0.1ug/ml dilution + Human enterovirus 71 (strain USA/BrCr/1970) (EV71) VP0 Protein, His Tag at 400ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 65 kDa 12% Bis-Tris Protein Gel.
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