Product Details
Source
Monoclonal Anti-TNF-alpha Antibody, Mouse IgG1 (16H5) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
16H5
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant Human TNF-alpha is expressed from human HEK293 cells
Specificity
This product is a specific antibody specifically reacts with TNF-alpha, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, IL-15,IL-21,GM-CSF and IFNγ.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA

Immobilized Human TNF-alpha, His Tag (active trimer) (Cat. No. TNA-H5228) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-TNF-alpha antibody, Mouse IgG1 (16H5) (Cat. No. TNA-Y59) with a linear range of 0.001-0.078 μg/mL (QC tested).
Protocol
Bioactivity-SPR

Monoclonal Anti-TNF-alpha antibody, Mouse IgG1 (16H5) (Cat. No. TNA-Y59) captured on CM5 chip via anti-mouse antibodies surface can bind Human TNF-alpha, His Tag (active trimer) (Cat. No. TNA-H5228) with an affinity constant of 0.274 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment

Background Introduction
Frontier Progress
- English Name:
Tumor necrosis factor α
- Category:
- Listed Drugs Count:
68 Details
- Clinical Drugs Count:
74 Details
- Highest R&D Stage:
Approved



















