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Product Details
Source
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AS113) (Omicron Specific) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with omicron Spike RBD. The antibody is specific against the Omicron (B.1.1.529/BA.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus and other viral lineages.
Antibody Type
Recombinant Monoclonal
Clone
AS113
Isotype
Human IgG1, Kappa
Immunogen
Recombinant SARS-CoV-2 Spike RBD (B.1.1.529/Omicron) erived from human 293 cells
Specificity
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1, has not been tested.
Application
ApplicationRecommended UsageELISA0.2-2 μg/mLPurification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA
Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No.SPD-C522e) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M415) with a linear range of 0.05-1.56 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No.SPD-C522e) and SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) and SARS-CoV-2 Spike RBD, His Tag (BA.1+L452R/Omicron) (Cat. No. SPD-C522k) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (AS113) (Omicron Specific) (Cat. No. SPD-M415) with a linear range of 0.049-3.125 ng/mL. The antibody does not bind SARS-CoV-2 Spike RBD WT (Cat. No. SPD-C52H3), Delta (Cat. No. SPD-C52Hh), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr), Alpha (Cat. No. SPD-C52Hn) (Routinely tested).
Protocol
User Reviews Publish Comment
B***a2023-02-28
LikesI used this antibody as a control for flow cytometry and it was very reliable. A clear peak shift could be seen with this antibody. When we had questions about what SARS-CoV-2 variants this mAb bound to, ACRO replied quickly and gave us information on the variants we inquired about and then some. ACRO is a company I would purchase from again and my company has used them consistently.- B***a2023-02-28 LikesI used this antibody as a control for flow cytometry and it was very reliable. When we had questions about what SARS-CoV-2 variants this mAb bound to ACRO replied quickly and gave us information on the variants we inquired about and then some. ACRO is a company I would purchase from again.
E***e2022-05-24 LikesThis omicron protein is a really great product and is high quality. The protein came on time to conduct our experiments and we were able to produce satisfactory results from using this protein. The protein was shipped with dry ice and was in a secure package. We would highly recommend this company and this protein to other labs that are doing omicron research.
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