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Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (MALS verified)

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Product Number/Spec
Price
Menge
SPD-M400a-100ug
$450.00
SPD-M400a-1mg (500ug X 2)
$3255.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Source

    The antibody is isolated from the serum of COVID-19 vaccine recipient and is recombinantly produced from human 293 cells (HEK293). This antibody can broadly neutralize SARS-CoV-2 Variants of Concerns (VOCs) including Alpha, Beta, Gamma, Delta and Omicron.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AM359b

  • Isotype

    Human IgG2, Kappa

  • Specificity

    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1 has not been tested.

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • Bioactivity-ELISA

     Spike RBD ELISA

    Immobilized SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) (Cat. No. SPN-C52Hz) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat. No. SPD-M400a) with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol
  •  Spike RBD ELISA

    Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat. No. SPD-M400a) with a linear range of 0.4-6 ng/mL (Routinely tested).

    Protocol
  •  Spike RBD ELISA

    Immobilized SARS-CoV-2 Spike protein WT (Cat. No. SPN-C52H9), Alpha ( Cat. No. SPN-C52H6), Beta (Cat. No. SPN-C52Hk), Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He) and Omicron (Cat. SPN-C52Hz) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat. No. SPD-M400a) with a linear range of 0.20-6.25 ng/mL (Routinely tested).

    Protocol
  •  Spike RBD ELISA

    Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat.No. SPD-M400a) neutralizes SARS-CoV-2 Spike Trimer by inhibiting Spike: ACE2 interaction. The Wild type (WT) Spike Trimer or Alpha, Delta, B.1.1.529/Omicron, BA.2/Omicron, BA.3/Omicron mutant-coated plate is incubated with the Biotinylated Human ACE2 / ACEH Protein and treated with the neutralizing antibody at increasing concentration . Percent inhibition is calculated based on the OD value.

    Protocol

User Reviews
Publish Comment

Background Introduction

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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