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Product Details
Source
Monoclonal Anti-SN38 Antibody, Mouse IgG1 is a recombinant mouse monoclonal antibody expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Isotype
Mouse IgG1, Kappa
Immunogen
SN38-OVA
Specificity
Specifically recognizes the target-SN38.
Application
ApplicationRecommended UsageELISA1:800-1:8000Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Data Display
Bioactivity-ELISA
Immobilized Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) at 5 μg/mL, add Sacituzumab Govitecam in the 50% Mouse serum and then add Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Sacituzumab govitecam at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) with a linear range of 0.12-0.98 ng/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) with Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) (Jackson, Cat. No. 109-035-098) used at 1:12000 concentration.
Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) is specific to Sacituzumab Govitecam and has no cross-reactivity with Trastuzumab Deruxtecan, Trastuzumab MMAE and Trastuzumab-DM1 (Routinely tested).
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