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Product Details
Source
Polyclonal Olig2 Antibody, Rabbit IgG is a polyclonal antibody purified from rabbit serum.
Gene Synonyms: Oligo2, bHLHe19, oligodendrocyte transcription factor 2.Antibody Type
Polyclonal Antibody
Clone
AS456
Isotype
Rabbit IgG, Kappa
Host Species
Rabbit polyclonal antibodies
Immunogen
Mouse Olig2 peptide fused to GST
Specificity
Usually, it is easier to get a positive signal in sections of juvenile animals than that of adults. This antibody does not recognize Olig1 and Olig3.
Application
ApplicationRecommended UsageIF1:200Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Liquid
Formulation
Supplied a 0.22 μm-filtered solution in PBS containing 50 mM Glycine and 50 mM Tris, with 40% glycerol as protectant.
Please contact us for customized product forms or formulations.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Shipped at -20°C. Store at -70°C for 12 months.
Notices
Product Specific Notices: For research use only.
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Immunostaining
2D cell staining: Immunofluorescent staining (10X) of cerebral organoid-derived neurons (CIPO-BWL001K) labeling Olig2 (Red) with purified OL2-S456 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
3D organoid staining: Immunofluorescent staining (4x or 10X) of cerebral organoid-(CIPO-BWL001K) labeling Olig2 (Green) with purified OL2-S456 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain. TUJ1 (red) is a neural differentiation marker commonly used to label neural processes.
User Reviews Publish Comment
Background Introduction
Zhang K, et al. Nat Commun. 2022;13(1):1423. doi: 10.1038/s41467-022-29068-z.
Ono K, et al. Mol Cells. 2009;27(4):397-401. doi: 10.1007/s10059-009-0067-2.
General Notes: FOR RESEARCH USE ONLY.
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