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Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Nucleocapsid Protein. This chimeric antibody is purified by Protein A affinity chromatography. As verified by binding test with N-NTD (Cat. No. NUN-C5143) and N-CTD (Cat. No. NUN-C5145) protein, this antibody can only bind to N-CTD (AA Ser 255 - Pro 364). ELISA test validated that this antibody can potently recognize the N protein variant with mutation located within CTD (Cat. No. NUN-C52H9). It can also bind multiple N protein variants with similar affinity as compared to the wild type N protein (Cat. No. NUN-C5227).
Human IgG1/kappa
This product can recognize SARS-CoV-2 Nucleocapsid protein. Cross-reactivity with Nucleocapsid protein of other coronaviruses has not been tested.
This antibody can be paired with other Anti-SARS-CoV-2 nucleocapsid antibodies to detect SARS-CoV-2 nucleocapsid protein in sandwich ELISA or LFA assay.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Less than 1.0 EU per μg by the LAL method.
This product is supplied and shipped with blue ice, please inquire the shipping cost.
Supplied as 0.2 μm filtered solution in PBS, pH7.4.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.