COA
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Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS95) is isolated from a SARS-CoV-2 infected patient and is recombinantly produced from human 293 cells (HEK293). This monoclonal antibody is purified by Protein A affinity chromatography. As verified by binding test with N-NTD (Cat.No. NUN-C5143) and N-CTD (Cat.No. NUN-C5145) protein, this antibody can only bind to N-NTD (AA Gly 44 - Glu 174). ELISA test validated that this antibody can bind multiple N protein variants with similar affinity as compared to the wild type N protein (Cat.No. NUN-C5227).
Human IgG1/kappa
This product can recognize SARS-CoV-2 Nucleocapsid protein. Cross-reactivity with Nucleocapsid protein of other coronaviruses has not been tested.
This antibody can be paired with other Anti-SARS-CoV-2 nucleocapsid antibodies to detect SARS-CoV-2 nucleocapsid protein in sandwich ELISA or LFA assay.
>95% as determined by SDS-PAGE.
Less than 1.0 EU per μg by the LAL method.
This product is supplied and shipped with blue ice, please inquire the shipping cost.
Supplied as 0.2 μm filtered solution in PBS, pH7.4 .
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.