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Product Details
Source
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
1M1E12
Isotype
Rabbit IgG, Kappa
Immunogen
MMAF-BSA
Specificity
Specifically recognizes MMAF and does not recognize MMAE.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Data Display
Bioactivity-ELISA
Immobilized IgG1-MMAF at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Serial dilutions of MMAF were added into Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214): IgG1-MMAF binding reactions. The half maximal inhibitory concentration (IC50) is 0.1106 μg/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214) with Doxorubicin-ADC, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 conju-gated antibody respectively.
The coating antibody was Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214), used at 1 μg/mL concentration. The primary an-tibody were different payload conjugated antibodies, including Doxorubicin-ADC, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 conjugated antibodies used at 0.25 μg/mL con-centration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Anti-body (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214) is specific to IgG1-MMAF and has no cross-reactivity with Doxorubi-cin-ADC, Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 (Routinely tested).
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