Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12)

Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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MME-MY2211-100ug
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MME-MY2211-1mg (500ug X 2)
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Source

Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) is a recombinant rabbit monoclonal antibody expressed in HEK293 cells.

Antibody Type

Recombinant Monoclonal

Clone

M1D12

Isotype

Rabbit IgG, Kappa

Host Species

Rabbit

Immunogen

MMAE-BSA

Specificity

Specifically recognizes MMAE and does not recognize MMAF.

Application
Application
Recommended Use
ELISA
0.2-2 μg/mL

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Purification

Protein A purified.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

Background Introduction

Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody specially react with MMAE without MMAF, which is more sensitive than mouse antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.