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Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (MALS verified)

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Product Number/Spec
Price
Menge
IL5-M542-100ug
$355.00
IL5-M542-1mg (200ug X 5)
$2345.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Source

    Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 is a recombinant mouse monoclonal antibody expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    12B11E5

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Recombinant Human IL-15 is expressed from human HEK293 cells

  • Specificity

    This product is a specific antibody specifically reacts with IL-15, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, IL-21,GM-CSF,TNF-alpha and IFNγ.

  • Application

    Application
    Recommended Usage
    ELISA
    1:200-1:2000
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • Bioactivity-ELISA

     IL-15 ELISA

    Immobilized Human IL-15, His Tag (Cat. No. IL5-H52H8) at 1 μg/mL (100 μL/well) can bind Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (Cat. No. IL5-M542) with a linear range of 0.2-4 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     IL-15 SPR

    Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (Cat. No. IL5-M542) captured on CM5 chip via anti-mouse antibodies surface can bind Human IL-15, His Tag (Cat. No. IL5-H52H8) with an affinity constant of 0.138 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.

Frontier Progress

 
Drug Development Progress
  • English Name:

    Interleukin-15

  • Category:

  • Listed Drugs Count:

    0 Details

  • Clinical Drugs Count:

    29 Details

  • Highest R&D Stage:

    Phase 2 Clinical

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