PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) (0.03% Proclin)

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Source

PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.

Antibody Type

Recombinant Monoclonal

Clone

W6/32

Isotype

Mouse IgG2a, Kappa

Host Species

Mouse

Reactivity

Human

Immunogen

Purified HLA class I Protein

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Specificity

This product is a specific antibody specifically reacts with HLA class I protein.

Application

Flow Cytometry (Evaluation of the expression of HLA class I on Human cells).

Recommended Dilution

1:50

Isotype Control

The isotype control is sold separately; please refer to Cat. No. DNP-PM487 for product information.

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
  • 2-8 °C for 12 months after reconstitution.
Notices

Product Specific Notices: For research use only.

Background Introduction

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.