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Product Details
Source
PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Clone
W6/32
Isotype
Mouse IgG2a, Kappa
Host Species
Mouse
Reactivity
Human
Immunogen
Purified HLA class I Protein
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Specificity
This product is a specific antibody specifically reacts with HLA class I protein.
Application
Flow Cytometry (Evaluation of the expression of HLA class I on Human cells).
Recommended Dilution
1:50
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-PM487 for product information.
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-Flow Cytometry
Flow cytometric analysis of Human peripheral blood lymphocytes staining with PE-Labeled Monoclonal Anti-HLA class I Antibody, Mouse IgG2a (W632) (Cat. No. HLI-PCFM816) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 PBMCs in a final volume of 100 µL), compared with PE Mouse IgG2a, k lsotype Ctrl(FC) Antibody. PE signal was used to evaluate the binding activity (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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