HRP conjugated Monoclonal Anti-His Antibody, Human IgG1 (AY63)

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HIS-PLM535-100ug
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HIS-PLM535-1mg (250ug X 4)
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Source

HRP conjugated Monoclonal Anti-His Antibody, Human IgG1 (AY63) is recombinantly expressed in HEK293. It consists of mouse variable regions genetically fused to human constant domains.

Antibody Type

Recombinant Monoclonal

Clone

AY63

Isotype

Human IgG1, Kappa

Host Species

Mouse

Reactivity

Tag

Immunogen

A protein peptide with his tag

Conjugate

HRP conjugated

Specificity

This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Application
Application
Recommended Use
Western Blot
0.2-2 ug/mL
ELISA
1:400-1:4000

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Purification

Protein A purified.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70 °C for 6 months after reconstitution.
  • 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

Background Introduction

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.