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Product Details
Source
Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
55F8
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Tag
Immunogen
A protein peptide with his tag
Conjugate
Acridinium ester Labeled
Specificity
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 15%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS, pH6.3, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Data Display
Bioactivity-MPCLIA
Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable at 37°C for 48 hours.
Protocol
The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable after freezing and thawing 3 times.
Protocol
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