HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016)

Cat. No. / Size
Price
Availability
Qty
G4S-HLY25G1-25ug
$410.00
In Stock - Delivery in 2-3 business days
G4S-HLY25G1-200ug
$1865.00
In Stock - Delivery in 2-3 business days

Product Details

  • Source

    Chimeric HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is recombinantly expressed in HEK293. It consists of rabbit variable regions genetically fused to rabbit IgG constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    016

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Conjugate

    HRP conjugated

  • Application

    Application
    Recommended Use / Performance
    ELISA
    1:1000-1:10000
  • Purification

    Protein A purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.01% PF68, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     G4S linker ELISA

    HRP-conjugated Monoclonal Anti-G4S Linker Antibody, Rabbit IgG (016) (Cat. No. G4S-HLY25G1) was used at 0.05 μg/mL for detection. Four recombinant proteins and one antibody containing different tandem repeats of G4S/G3S peptide linkers, including (G4S)₂, (G4S)₃, (G3S)₂, (G3S)₃, and (G3S)₄, were tested. Dose-dependent binding was observed for all tested G4S/G3S linker-containing samples by ELISA (QC tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background

The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.

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