HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016)

Cat. No. / Size
Price
Qty
G4S-HLY25G1-25ug
$410.00
G4S-HLY25G1-200ug
$1865.00
ETA of in-stock products:2 business days

Product Details

  • Source

    HRP conjugated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a recombinant rabbit monoclonal antibody expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    016

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Conjugate

    HRP Labeled

  • Application

    Application
    Recommended Usage
    ELISA
    1:1000-1:10000
  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.01% PF68, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     G4S linker ELISA

    HRP-conjugated Monoclonal Anti-G4S Linker Antibody, Rabbit IgG (016) (Cat. No. G4S-HLY25G1) was used at 0.05 μg/mL for detection. Four recombinant proteins and one antibody containing different tandem repeats of G4S/G3S peptide linkers, including (G4S)₂, (G4S)₃, (G3S)₂, (G3S)₃, and (G3S)₄, were tested. Dose-dependent binding was observed for all tested G4S/G3S linker-containing samples by ELISA (QC tested).

    Protocol

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Background

The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.

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  • Background