Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1)

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Cat. No. / Size
Price
Availability
Qty
DON-MY2215-100ug
$410.00
In Stock - Delivery in 2-3 business days
DON-MY2215-1mg (500ug X 2)
$2455.00
In Stock - Delivery in 2 weeks

Product Details

  • Source

    Chimeric Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) is recombinantly expressed in HEK293. It consists of rabbit variable regions genetically fused to rabbit IgG constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    1M2B1

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Immunogen

    Doxorubicin-BSA

  • Specificity

    Specifically recognizes Doxorubicin.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Doxorubicin ELISA

    Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (Cat. No. DON-MY2215) with a linear range of 0.06-8 ng/mL (QC tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background

Doxorubicin a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. As the payload of ADC drugs, Doxorubicin can act directly on DNA, insert into the double helix strands of DNA, cause the latter to unwind, change the template properties of DNA, and inhibit DNA polymerase, thereby inhibiting the synthesis of both DNA and RNA. Anti-Doxorubicin antibody is a rabbit monoclonal antibody specifically reacts with Doxorubin without other payloads, which is more sensitive than mouse antibody. The anti-Doxorubicin antibody is a useful reagent in PK assay to determine conjugated antibodies.

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