Recombinant Monoclonal Anti-CK20 Antibody, Rabbit (5D4)

Product Details

Background
Recombinant Monoclonal Anti-CK20 Antibody, Rabbit (5D4) is a CK20 primary antibody for IHC application. CK20 belongs to cytokeratin family and typically expressed in the epithelial cells of the gastrointestinal and urinary tracts. CK20 serves as a critical tumor biomarker in the clinical practice in a variety of tumors in the colon and bladder.

Host Species	Rabbit
Isotype	Rabbit IgG, Rabbit Kappa
Clone	5D4
Application	IHC-P
Dilution Ratio	1: 200 - 1: 800
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	Cytokeratin 20
Concentration	0.9~1.1 mg/ml
Specificity	React with CK20 protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	PBS, BSA, Glycerol

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human colorectal cancer tissue labelled with HGS-S602 at 1:400 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human urothelial carcinoma tissue labelled with HGS-S602 at 1:400 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.