PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (0.03% Proclin)

The binding activity of PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) to different humanized VHHs was evaluated by ELISA. Humanized VHHs were coated at 1 μg/mL. The antibody was serially diluted starting at 125 ng/mL, followed by incubation with an HRP-conjugated goat anti-rabbit IgG Fc-specific secondary antibody at a 1:30,000 dilution. Absorbance was measured at 450 nm (Routinely tested).

The binding activity of PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) to different alpaca VHHs was evaluated by ELISA. Alpaca VHHs were coated at 1 μg/mL. The antibody was serially diluted starting at 62.5 ng/mL, followed by incubation with an HRP-conjugated goat anti-rabbit IgG Fc-specific secondary antibody at a 1:30,000 dilution. Absorbance was measured at 450 nm (Routinely tested).

Flow cytometric analysis of Anti-BCMA CAR (CQ)-293 Stable Cell Line stained with PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) at a 1:50 dilution (2 μL of antibody stock solution was used to stain 1 × 10⁶ cells in a final volume of 100 μL), with an isotype control antibody included as a negative control. PE fluorescence signals was used to evaluate binding activity (QC tested).

Flow cytometric analysis of the non-specific binding of PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (Cat. No. CAH-PMY2491a) to CD3+ cells in human PBMCs. A total of 5 × 10⁵ human PBMCs were stained with FITC-labeled anti-CD3 antibody alone (left) or co-stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-Camelid VHH Antibody, Rabbit IgG (M1A11) (right) (2 μL of antibody stock solution per 5 × 10⁵ cells in a final staining volume of 100 μL), washed, and analyzed by FACS. FITC and PE fluorescence signals were used to evaluate the non-specific binding of the PE-labeled antibody to human CD3+ cells (QC tested).