Alexa Fluor™ 555-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (0.03% Proclin)

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Source

Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) is a non-neutralizing antibody expressed from human HEK293 cells, which provides higher batch consistency and long term security of supply.

Clone

3G8C1

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Mouse

Immunogen

Recombinant C11D5.3 scFv derived from HEK293 cells

Conjugate

AF555

Excitation Wavelength: 561 nm

Emission Wavelength: 572 nm

Specificity

Specifically recognizes C11D5.3 derived CARs.

Application
Application
Recommended Use
Flow Cytometry
1:50

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
  • 2-8 °C for 12 months after reconstitution.
Notices

Product Specific Notices: For research use only.

Background Introduction

C11D5.3 is an IgG1 mouse monoclonal antibody specific for BCMA, which is a target for the immunotherapy of multiple myeloma and lymphomas. C11D5.3 scFv is the most commonly used ectodomain componse of BCMA-specific CARs. So far, multiple reported CART BCMA trials contain the anti-BCMA scFv derived from C11D5.3, such as FDA approved CARs Abecma.

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