Alexa Fluor™ 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (0.03% Proclin)

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218-AFFMY3-25tests
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Source

Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.

Antibody Type

Recombinant Monoclonal

Clone

P3C09

Isotype

Rabbit IgG, Kappa

Host Species

Rabbit

Reactivity

All

Immunogen

Purified 218 linker protein

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Specificity

This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.

Application
Application
Recommended Use
Flow Cytometry
1:25

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
Notices

Product Specific Notices: For research use only.

Background Introduction

The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.

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