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Product Details
Product Details
Human iPSC-Derived Dorsal Forebrain Organoids (Cat. No. CIPO-BWL02) are three-dimensional in vitro models that recapitulate key developmental and cellular features of the human dorsal forebrain. These organoids are generated from the human iPSC line ATCC-HYR0103 under ultra-low attachment (ULA) culture conditions using a self-assembly differentiation workflow. Human iPSC-Derived Dorsal Forebrain Organoids exhibit characteristic mature dorsal forebrain and cortical features, including MAP2-positive neurons, GFAP-positive astrocytes, OLIG2-positive oligodendroglial lineage cells, and spontaneous electrical activity. The organoids are shipped at ambient temperature in a ready-to-use format and can be maintained using the Human iPSC-Derived Dorsal Forebrain Organoid Maintenance Kit (Cat. No. RIPO-BWM01). This model is suitable for a range of applications, including studies of human brain development, neurological disease modeling, neurotoxicity assessment, neuronal excitability and network activity studies, and other neuroscience research.
Product Specification
Ready-to-use hiPSC-Derived Dorsal Forebrain Organoids are delivered in a specialized shipping medium and require a short recovery period before downstream assays or experiments.
Storage
Upon receipt, transfer the organoids to fresh maintenance medium as soon as possible and follow the appropriate incubation conditions and medium change schedule.
Shipping
This product is supplied and shipped with blue ice, please inquire the shipping cost.
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Performance Data
Product Diagram
Workflow for dorsal forebrain organoid differentiation.
Marker Expression
Marker Expression in hiPSC-Derived Dorsal Forebrain Organoids
At Day 27, the organoids expressed PAX6, TUJ1, and FOXG1 with minimal NKX2.1 expression, supporting a dorsal forebrain identity. By Day 57–87, the organoids showed progressive neuronal maturation and cellular diversification, with CTIP2- and MAP2-positive neurons, vGLUT1-positive excitatory neurons, GFAP-positive astrocytes, and OLIG2-positive cells.
ProtocolOrganoid Activity
Intra-batch morphological consistency
Organoids within the same batch showed consistent growth and morphology over time, with diameter CVs (QC) ranging from approximately 4% to 15%, demonstrating good intra-batch consistency.
Inter-batch morphological consistency
Organoids from three independent batches showed similar growth patterns and morphological (QC) changes, with consistent sizes across batches, demonstrating good inter-batch consistency.
ProtocolProtocolOrganoid Application
Spontaneous calcium activity in dorsal forebrain organoids
Dorsal forebrain organoids exhibited spontaneous, rhythmic calcium transients over a 180-s recording period using the FLIPR Calcium 6 assay, with clear periodic ΔF/F₀ oscillations (in video).
Electrophysiological profiling of hiPSC-derived dorsal forebrain organoids by MEA
All 5/5 dorsal forebrain organoids exhibited robust spontaneous electrophysiological activity (QC), with clear waveforms recorded over a 6-min MEA measurement period (in video).
Compound-Induced Neuronal Hyperexcitability
Glutamate and Yoda1 treatment increased neuronal activity compared with baseline, with higher spike numbers, more active electrodes, and denser spiking patterns. Yoda2 produced a stronger response, particularly in firing rate, demonstrating that MEA-based brain organoids can capture compound-induced neuronal hyperexcitability and support neuroactive compound screening.
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